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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (1993) 17, 205–212 (Printed in Great Britain)
Expression of the 30 kD cysteine endoprotease B in germinating barley seeds
Salla Marttila, Ilkka Porali, T.‑H.David Ho and Anita Mikkonen
Biol. Dept., Univ. Jyväskylä, POB 35, SF-40351 Jyväskylä, Finland, and Biol. Dept., Washington Univ., St. Louis, MO 63130, USA


Abstract

The expression of a 30 kD cysteine endoprotease (EP-B) was studied by in situ hybridization and immunomicroscopy to clarify its role in germinating barley grains. At the beginning of germination, EP-B mRNA was expressed in the scutellar epithelium and aleurone cells next to the embryo. Later, mRNA levels were highest in the aleurone layer proceeding to the distal end of the grain. During the first day of germination, EP-B protein was strongly localized to the germ aleurone and scutellar epithelium from where the secretion into the starchy endosperm began. Secretion was also observed to proceed along the aleurone layer to the distal end. These results show that EP-B is differentially localized during germination, and both scutellum and aleurone layer are able to synthesize and secrete EP-B protein.



doi:10.1006/cbir.1993.1056


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)