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Cell Biology International (1993) 17, 953960 (Printed in Great Britain)
Modulation of glycolysis induction in primary cultures of rabbit kidney proximal tubule cells: the role of shaking, glucose and insulin.
Christelle Monteil, Caroline Leclere, Françoise Dantzer, Veronika Elkaz, Jean‑Paul Fillastre and Jean‑Paul Morin
INSERM U-295-Universite de Rouen, B.P. 97, 76800 Saint Etienne du Rouvray, France.
We have assessed the impact of increasing oxygen availability on cellular phenotype expression of rabbit proximal tubule cells in primary culture developed with variable glucose and/or insulin contents. To mitigate hypoxia at the cell/medium interface, cells were shaken for the whole culture duration and their expressed phenotype was compared with those expressed by static cultures. O