|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (1995) 19, 833838 (Printed in Great Britain)
Secretion of TNF-α from macrophages following induction with a lignin derivative.
Kenji Sorimachi, Kazumi Akimoto, Keiko Tsuru, Shigeki Nagashima, Tamio Ieiri and Akira Niwa
Department of Microbiology, Laboratory of Molecular and Cellular Biology, Department of Ophthalmology, Department of Clinical Pathology, Dokkyo University School of Medicine, Mibu, Tochigi 321-02, Japan.
Macrophages derived from rat bone marrow were treated with macrophage colony stimulating factor (M-CSF) to obtain a sufficient number of cells for the tumor necrosis factor (TNF-α) assay. The present study has been designed to investigate whether the production of TNF-α, which induces multinucleated giant cell formation, is regulated by polyanions such as lignin derivatives. ELISA for TNF-α showed that the polyanion induced TNF-α production by macrophages. The secretion of TNF-α from the cells reached a maximum at 3-6 h, and then showed a slight decline. Northern blotting of TNF-α mRNA showed that the amount of TNF-α reached a maximum within 1 h of macrophage culture in the presence of a lignin derivative. On the other hand, TNF-α mRNA was undetectable in the control cells. It was concluded that stimuli such as that provided by lignin derivatives increases the amount of TNF-α mRNA, which is then followed by translation of TNF-α.