|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (1995) 19, 839846 (Printed in Great Britain)
Effect of extracellular matrix proteins on vascular smooth muscle cell phenotype
I.P. Hayward, K.R. Bridle, G.R. Campbell, P.A. Underwood and J.H. Campbell
Centre for Research in Vascular Biology, Department of Anatomical Sciences, University of Queensland, Qld 4072, Australia and CSIRO Division of Biomolecular Engineering, Sydney Laboratory, PO Box 184, North Ryde, NSW 2113, Australia.
The effect on phenotypic expression of rabbit vascular smooth muscle cells (SMC) of the interstitial matrix proteins collagen I and fibronectin, the basal lamina proteins collagen IV and laminin, and the serum adhesion protein vitronectin was examined in culture. Experiments were performed in foetal calf serum stripped of fibronectin and vitronectin to eliminate their confounding effects. All the proteins promoted adhesion to the plastic culture dish (in a concentration dependent manner) of SMC freshly isolated from the artery wall. These cells had a high volume density of myofilaments (V