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Cell Biology International (1996) 20, 7–14 (Printed in Great Britain)

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A NOVEL METHOD FOR PRODUCTION OF MONOCLONAL ANTIBODIES. EVALUATION AND EXPECTATION OF THE RAT LYMPH NODE METHOD IN CELL AND MOLECULAR BIOLOGY

YOSHIKAZU SADO and TOHRU OKIGAKI

Divisions of Immunology, and Molecular and Cell Biology, Shigei Medical Research Institute, 2117 Yamada, Okayama 701-02, Japan

Abstract

A newly developed method using rat lymph nodes provided us with substantial amounts of high quality monoclonal antibodies. The method has characteristic features, a single injection of an emulsified antigen via the rat’s hind footpads is sufficient for immunization. Enlarged medial iliac lymph nodes can be used for cell fusion to produce hybridomas 2 weeks and later after the antigen injection. The production frequency of target hybridomas is about 10 times higher than that of a conventional spleen method. Our current knowledge about the production of monoclonal antipeptide antibodies and monoclonal nephritogenic autoantibodies by the method is described.

Key words: monoclonal antibody, antipeptide antibody, type IV collagen, basement-membrane collagen, rat lymph node.