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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (1996) 20, 351–354 (Printed in Great Britain)
HUMAN SPERM β-GLUCURONIDASE IS POORLY EXTRACTABLE BY TRITON X-100
ADRIANO BRANDELLIa*, PATRICIA V. MIRANDAb and JORGE G. TEZÓNb
aDepartamento de Bioquimica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, 90050-170, Porto Alegre, Brasil
bInstituto de Biologia y Medicina Experimental, CONICET, 1428, Buenos Aires, Argentina


Abstract

A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1MKCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3MKCl or DNAse I treatment. Results demonstrate that β-glucuronidase is tightly associated to the Triton X-100 resistant fraction.


Key words: glycosidase, enzyme, sperm, detergent, cytoskeleton.

f1To whom correspondence should be addressed.


doi:10.1006/cbir.1996.0040


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)