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Cell Biology International (1996) 20, 351–354 (Printed in Great Britain)

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HUMAN SPERM β-GLUCURONIDASE IS POORLY EXTRACTABLE BY TRITON X-100

ADRIANO BRANDELLI^a*, PATRICIA V. MIRANDA^b and JORGE G. TEZÓN^b

^aDepartamento de Bioquimica, Instituto de Biociências, Universidade Federal do Rio Grande do Sul, 90050-170, Porto Alegre, Brasil
^bInstituto de Biologia y Medicina Experimental, CONICET, 1428, Buenos Aires, Argentina

Abstract

A part of sperm glycosidase activities was detected as detergent-insoluble after sequential extractions with Triton X-100. Sixty per cent of total β-glucuronidase activity was found in the detergent-insoluble fraction. This portion of β-glucuronidase was resistant to extractions in the presence of 1MKCl, chaotropic agents, colchicine or cytochalasine B, being only partially solubilized by 3MKCl or DNAse I treatment. Results demonstrate that β-glucuronidase is tightly associated to the Triton X-100 resistant fraction.

Key words: glycosidase, enzyme, sperm, detergent, cytoskeleton.

^f1To whom correspondence should be addressed.