|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (1997) 21, 229236 (Printed in Great Britain)
KINESIN-ASSOCIATED TRANSPORT IS INVOLVED IN THE REGULATION OF CELL ADHESION
IRINA N. KAVERINAa,f1, ALEXANDER A. MININb, FATIMA K. GYOEVAb and JURY M. VASILIEVa
aCancer Research Center, Russian Academy of Medical Sciences, Kashirskoe sh. 24, Moscow, 115478
bInstitute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, 142292, Russia
It has been recently shown that depolymerization of microtubules induces the elongation of focal contacts at the leading edge. On the other hand, cell shape and pseudopodial activity were found to depend on the microtubule-based motor kinesin. In this paper, we examine whether kinesin is involved in controlling the dynamics of adhesive structures at the cell surface. Microinjection of an antiblocking kinesin activityin vitrocauses focal contact elongation similar to the effect of microtubule-depolymerizing drugs. Thus, the role of microtubules in cell adhesion lies in the supporting kinesin-based transport to the adhesion sites.
Key words: Focal adhesion, kinesin, microtubules.
f1To whom correspondence should be addressed: (present address) Institute of Moelcular Biology, Austrian Academy of Sciences, Billrothstr. 11, A/5020, Salzburg, Austria