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Cell Biology International (1997) 21, 243–248 (Printed in Great Britain)

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EVIDENCE FOR A LOCALIZATION SIGNAL IN THE 3′UNTRANSLATED REGION OF MYOSIN HEAVY CHAIN MESSENGER RNA

JOHN W. WISEMAN^ab, L.ANNE GLOVER^a and JOHN E. HESKETH^b,f1

^aDepartment of Molecular and Cell Biology, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD
^bIntracellular Targeting Group, Rowett Research Institute, Bucksburn, Aberdeen, AB21 9SB, U.K.

Abstract

Localization signals in the 3′untranslated region (3′UTR) of myosin heavy chain mRNA were investigated using hybrid gene constructs. In myoblasts transfected with constructs containing either both coding sequences and 3′UTR of the rabbit β-globin gene or the β-globin coding sequences alonein situhybridization showed that globin transcripts were distributed throughout the cytoplasm with no localization. In contrast, in myoblasts transfected with β-globin coding sequences linked to the myosin heavy chain 3′UTR there was strong perinuclear localization of the hybrid mRNA; this was maintained in myotubes. We conclude that myosin heavy chain 3′UTR contains a localization signal.

Key words: myosin, targeting, 3′untranslated region, messenger RNA, post-transcriptional control, myoblasts.

^f1To whom correspondence should be addressed