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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (1997) 21, 243–248 (Printed in Great Britain)
EVIDENCE FOR A LOCALIZATION SIGNAL IN THE 3′UNTRANSLATED REGION OF MYOSIN HEAVY CHAIN MESSENGER RNA
JOHN W. WISEMANab, L.ANNE GLOVERa and JOHN E. HESKETHb,f1
aDepartment of Molecular and Cell Biology, Institute of Medical Sciences, Foresterhill, Aberdeen, AB25 2ZD
bIntracellular Targeting Group, Rowett Research Institute, Bucksburn, Aberdeen, AB21 9SB, U.K.


Abstract

Localization signals in the 3′untranslated region (3′UTR) of myosin heavy chain mRNA were investigated using hybrid gene constructs. In myoblasts transfected with constructs containing either both coding sequences and 3′UTR of the rabbit β-globin gene or the β-globin coding sequences alonein situhybridization showed that globin transcripts were distributed throughout the cytoplasm with no localization. In contrast, in myoblasts transfected with β-globin coding sequences linked to the myosin heavy chain 3′UTR there was strong perinuclear localization of the hybrid mRNA; this was maintained in myotubes. We conclude that myosin heavy chain 3′UTR contains a localization signal.


Key words: myosin, targeting, 3′untranslated region, messenger RNA, post-transcriptional control, myoblasts.

f1To whom correspondence should be addressed


doi:10.1006/cbir.1997.0140


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)