Brought to you by Portland Press Ltd.
Published on behalf of the International Federation for Cell Biology
Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (1998) 22, 237–243 (Printed in Great Britain)
ALTERATIONS IN DISTRIBUTION OF ACTIN BINDING PROTEINS IN UTERINE STROMAL CELLS DURING DECIDUALIZATION IN THE RAT
TIMOTHY J SHAW, VERA TERRY, CEDRIC D SHOREY and CHRISTOPHER R MURPHY
Department of Anatomy and Histology and Institute for Biomedical Research, The University of Sydney, NSW, 2006, Australia


Abstract

Immunohistochemistry was used to investigate the involvement of the actin-associated binding proteins, tropomyosin, α-actinin and gelsolin with the formation of the decidual cell reaction during early pregnancy in the rat. Tropomyosin was present in the uterine myometrium, but absent from the both decidual and non-decidual stromal cells. α-Actinin was absent from non-decidual stromal cells, but present in decidual cells. Gelsolin was present in non-decidual cells close to the uterine stroma as well as in transformed decidual cells. Both gelsolin and α-actinin were concentrated around the periphery of the cell. It is proposed that these actin-binding proteins may be involved with the cellular transformations associated with decidualization.


Keywords: decidualization, actin-binding proteins, rat, uterus.

f1*To whom correspondence should be addressed: T. J. Shaw, Department of Anatomy and Histology, F13, University of Sydney, NSW, 2006, Australia.


References

1 Abrahamsohn, PA and Zorn, TMT (1983) Implantation and decidualization in rodents. J Exp Zool 266, 603-28

2 Andrade, CG, Savino, W, Abrahansohn, PA and Zorn, TMT (1994) Distribution of desmoplankin I/II in endometrial cells of mice in the artificially induced decidua. Tiss Cell 26, 639-47

3 Barclay, AN (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology 42, 593-600

4 Bennett, V and Gilligan, DM (1993) The spectrin-based membrane skeleton and micron-scale organization of the plasma membrane. Annu Rev Cell Biol 9, 27-66

5 Bishop, GA, Hall, BM, Duggin, GG, Horvath, JS, Ross Sheil, AG and Tiller, DJ (1986) Immunopathology of renal allograft rejection analysed with monoclonal antibodies to mono-nuclear cell markers. Kidney Int 29, 708-17

6 Carraway, KL (1990) Membranes and microfilaments: Interactions and role in cellular dynamics. Bioessays 12, 90-2

7 Carson, DD, Julian, J and Jacobs, AL (1992) Uterine stromal cell chondroitin sulfate proteoglycans bind to collagen type I and inhibit embryo outgrowth in vitro. Dev Biol 149, 307-16

8 Carter, CA, Rinehart, CA, Bagnell, CR and Kaufman, DG (1991) Fluorescent laser scanning microscopy of F-actin disruption in human. Pathobiology 59, 36-45

9 Christensen, S, Verhage, HG, Nowak, G, de Lanerolle, P, Fleming, S and Bell, SC (1995) Smooth muscle myosin II and alpha smooth muscle actin expression in the Baboon (Papio anubis. Biol Reprod 53, 598-608

10 Condeelis, J (1993) Life at the leading edge: the formation of cell protrusions. Annu Rev Cell Biol 9, 411-44

11 Cunningham, CC (1992) Actin structural proteins in cell motility. Cancer Metast Rev 11, 69-77

12 Dubreuil, RR (1991) Structure and evolution of the actin crosslinking proteins. Bioessays 13, 219-26

13 Finn, CA (1971) Biology of decidual cells. Adv Reprod Physiol 5, 1-26

14 Finn, CA (1994) . Implantation. Marshalls Physiology of Reproduction.

15 Finn, CA and McClaren, A (1967) A study of the early stages of implantation in the mouse. J Reprod Fert 13, 259-67

16 Finn, CA and Porter, DG (1975) . The Uterus.

17 Geiger, B, Dutton, AH, Tokuyasu, KT and Singer, SJ (1981) Immunoelectron microscope studies of membrane-microfilament interactions: distributions of α-actinin, tropomyosin and vinculin in intestinal epithelial brush border and chicken gizzard smooth muscle cells. J Cell Biol 91, 614-28

18 Glasser, SR, Lampelo, S, Munir, MI and Julian, J (1987) Expression of desmin, laminin and fibronectin during in situ differentiation (decidualization) of rat uterine stromal cells. Differentiation 35, 132-42

19 Hay, ED (1989) Extracellular matrix, cell skeletons, and embryonic development. [Review]. Am J Med Genet 34, 14-29

20 Hitt, LA and Luna, EJ (1994) Membrane interactions with actin cytoskeleton. Curr Opin Cell Biol 6, 120-30

21 Kranen, RW, Overes, HWTM, Kloosterboer, HG and Poels, LG (1993) The expression of cytoskeletal proteins during the differentiation of rat granulosa cells. Human Reprod 8, 24-9

22 Long, JA and Evans, HM (1922) The oestrus cycle of the rat and its associated phenomena. Mem Univ Calif 6, 1-148

23 Lundkvist, O and Ljungkvist, I (1977) Morphology of the rat endometrial stroma at the appearance of the pontamine blue reaction during implantation after an experimental delay. Cell Tiss Res 184, 453-66

24 Luxford, KA and Murphy, CR (1992) Reorganisation of the apical cytoskeleton of uterine epithelial cells during early pregnancy in the rat: a study with myosin subfragment 1. Biol Cell 74, 195-203

25 Maciver, SK, Wachsstock, DH, Schwarz, WH and Pollard, TD (1991) The actin filament severing protein acto-phorin promotes the formation of rigid bundles of actin filaments crosslinked with α-actinin. J Cell Biol 115, 1621-8

26 Mallein-Gerin, F, Garrone, R and van der Rest, M (1991) Proteoglycan and collagen synthesis are correlated with actin organisation in dedifferentiating chondrocytes. Eur J Cell Biol 56, 364-73

27 McLaughlin, PJ, Gooch, JT, Mannherz, HG and Weeds, AG (1993) Structure of gelsolin segment 1-actin complex and the mechanism of filament severing. Nature 364, 685-92

28 Parr, MB and Parr, EL (1986) Permeability of primary decidual zone in the rat uterus: Studies using fluorescein-labeled proteins and dextrans. Biol Reprod 34, 393-404

29 Payratre, B (1992) Cytoskeleton, phosphoinositides and signal transduction. Med Sci 8, 127-33

30 Pollard, TD and Cooper, JA (1986) Actin and actin-binding proteins. A critical evaluation of mechanics and functions. Annu Rev Biochem 55, 987-1035

31 Rao, KMK and Cohen, HJ (1991) Actin cytoskeletal network in aging and cancer. Mutat Res 256, 139-48

32 Shaw, TJ and Murphy, CR (1995) Leucocyte involvement in lectin-induced deciduomata formation. Cell Biol Int 19, 577-84

33 Shaw, TJ and Murphy, CR . Shaw, TJ, Terry, V, Shorey, CD, Murphy, CR, 1997, Alterations in actin distribution in the endometrium during implantation and decidualization in the rat

34 Small, JV (1982) Organisations of actin and fibroblast locomotion. Prog Clin Biol Res 85, 341-58

35 Stossel, TP, Chaponnier, C, Ezzell, RM, Hartwig, JH, Janmey, PA, Kwiatkowski, DJ et al. (1985) Nonmuscle actin-binding proteins. Annu Rev Cell Biol 1, 353-402

36 Terry, V, Shaw, TJ, Shorey, CD and Murphy, CR (1996) Actin binding proteins undergo major alterations during plasma membrane transformation in uterine epithelial cells. Anat Rec 246, 71-7

37 Trifaro, JM, Rodriguez del Castillo, A and Vitale, ML (1992) Dynamic changes in chromaffin cell cytoskeleton as prelude to exocytosis. Mol Neurobiol 6, 339-58

38 Tung, HN, Parr, MB and Parr, EL (1986) The permeability of the PDZ in the rat uterus. An ultrastructural tracer and freeze fracture study. Biol Reprod 35, 1045-58

39 Weeds, AG, Gooch, J, Hawkins, M, Pope, B and Way, M (1991) Role of actin-binding proteins in cytoskeletal dynamics. Biochem Soc Trans 19, 1016-20

40 Yin, HL and Stossel, TP (1979) Control of cytoplasmic actin gel-sol transformation by gelsolin, a calcium dependant regulatory protein. Nature 281, 583-6

41 Zafar, A and Thampan, RV (1995) Association of cytoskeletal proteins with estrogen receptor in rat uterine. Biochem Mol Biol Int 36, 1197-206

42 Zaner, KS and Hartwig, JH (1988) The effect of filament shortening on the mechanical properties of gel-filtered actin. J Biol Chem 263, 4532-6


Received 31 October 1997; accepted 16 March 1998

doi:10.1006/cbir.1998.0245


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)