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Cell Biology International (1998) 22, 131–135 (Printed in Great Britain)
EFFECTS OF ADENOSINE AND EPIDERMAL GROWTH FACTOR ON THE GROWTH OF MOUSE MAMMARY EPITHELIAL CELLS
I.S YUH, B.B JANG and B.J HONG
Department of Dairy Science, College of Animal Agriculture, Kangwon National University, Chuncheon, Korea, 200-701


Abstract

The objective of this study was to determine if adenosine alters growth of mammary epithelium. Mouse mammary epithelial cells (NMuMG) were cultured in DMEM supplemented with 10% fetal calf serum. After serum starvation for 24h, EGF (0–100ng/ml) and/or adenosine (0–100μm) was added. Adenosine at concentrations of 1, 10 or 100μmincreased DNA synthesis significantly, when compared to control. Addition of epidermal growth factor (EGF) (10ng/ml) into 1 or 10μmadenosine showed the interaction in DNA synthesis between EGF and adenosine. A similar result was observed when 100μmadenosine added to various concentrations of EGF (0–100ng/ml). In the second mammary gland (thoracic) organ culture studies, mammary development scores were increased by adenosine (100μm), EGF (100ng/ml) and adenosine plus EGF. These results indicate that the purine nucleoside adenosine stimulates mammary epithelial cell growth and interacts with EGF in DNA synthesis of mouse mammary epithelial cells.


Key words: adenosine, mammary epithelium, EGF, DNA synthesis.

f1*To whom correspondence should be addressed.


Received 13 October 1997; accepted 9 February 1998

doi:10.1006/cbir.1998.0231


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)