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Cell Biology International (1998) 22, 177183 (Printed in Great Britain)
DECREASED DENSITIES OF INTRAMEMBRANOUS PARTICLES AND CYTOCHEMICALLY DETECTABLE CHOLESTEROL IN MICROVILLI OF STARVED RAT ENTEROCYTES
A.A WAHEEDa, Y TOYAMAb, F YASUZUMIc and P.D GUPTAa,f1
aCentre for Cellular and Molecular Biology, Hyderabad, 500 007, India
bDept of Anatomy, Chiba University, Chiba, 280, Japan
c2nd Department of Anatomy, University of the Ryukyus, Okinawa, 903-01, Japan
The densities of intramembranous particles (IMPs) and of sterol complexes induced by treatment of filipin were studied by freeze-fracture replication of intact intestine and/or isolated brush border membranes (BBM) of well-fed and starved rats. The density of IMPs and filipin-sterol complexes (FSCs) decrease considerably during starvation. Biochemical estimations show a decrease in the levels of cholesterol and proteins with respect to phospholipids during starvation which is in agreement with morphological findings. It is suggested that these changes may play a role in regulating membrane fluidity which in turn affects absorption of nutrients through BBM.
Key words: rat intestine, lasting, brush border membranes, freeze-fracture, cholesterol, protein phospholipid ratio.
f1*To whom correspondence should be addressed.
Received 15 October 1997; accepted 23 January 1998doi:10.1006/cbir.1998.0227