|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (1998) 22, 227236 (Printed in Great Britain)
ANTHERIDIAL CHROMATIN CONDENSATION FACTOR FROM MALE SEX ORGANS OFCHARA TOMENTOSA
ANDRZEJ KAŹMIERCZAK and JANUSZ MASZEWSKI
Department of Cytophysiology, University of Łódź, 90-231, Łódź, ul. Pilarskiego 14, Poland
Incubation of roots (Melandrium noctiflorum) in crude extracts obtained from maturing male sex organs ofChara tomentosaresults in supercondensation of mitotic chromosomes. The observed changes, including a number of ‘secondary effects’, confirm precisely with all those kinds of alterations which are typical of M-phase cells in antheridial filaments during their progression from the proliferative period of development towards terminal differentiation into sperm cells. Biological assays performed with proteinaceous extracts of maturing antheridia indicate that the activity is confined merely to a fraction containing low molecular (4.5
Key words: chromosome supercondensation, spermatogenesis, Chara tomentosa, Melandrium noctiflorum, endocytosis.
Received 21 March 1997; accepted 11 March 1998doi:10.1006/cbir.1998.0242