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Cell Biology International (1999) 23, 227–233 (Printed in Great Britain)

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Cisplatin inhibits folic acid chemotaxis and phagocytotic functions in dictyostelium discoideum

T.B.K Reddy^f1 and S Chatterjee

School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067, India

Abstract

Administration of 100 and 200μg/ml of cisplatin [cis -diammine dichloro platinum (II)] for 1h to growing Dictyostelium discoideum cells severely affects folic acid chemotaxis and phagocytotic function in this organism. Following cisplatin treatment, cells show a much lower uptake of FITC labelled bacteria and a reduced plaque forming ability when plated on Eschericia coli seeded normal agar. Folic acid chemotaxis and folate deaminase activity are greatly inhibited in cisplatin-treated Dictyostelium cells. SDS-PAGE analysis shows a greater association of actin and myosin with the cell cortex of treated cells. These results have been discussed in relation to cisplatin's known ability to raise the levels of cytosolic calcium.

Key words: cisplatin, dictyostelium, chemotaxis, phagocytosis.

^f1*To whom correspondence should be addressed: Dr T. B. K. Reddy, Norris Cancer Center, Room 6429 University of Southern California 1441 Eastlake Avenue Mail stop 73 Los Angeles CA 90033 U.S.A. E-mail:treddy@usc.edu

Received 23 September 1998; accepted 25 November 1998