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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2000) 24, 175–181 (Printed in Great Britain)
PARTIAL CHARACTERIZATION OF MOSQUITO LARVAE EXTRACT INDUCING DNA SYNTHESIS ALTERATIONS ON HUMAN MONONUCLEAR CELLS
J.R. Ronderosa,f1, M.A. Salasb, O.J. Rimoldic and G. Finarellic
aCátedra de Histologı́a y Embriologı́a ‘B’ Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calle 60 y 120, 1900, La Plata, Argentina
bCátedra de Fisiologı́a con Biofı́sica, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calle 60 y 120, 1900, La Plata, Argentina
cINIBIOLP, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, Calle 60 y 120, 1900, La Plata, Argentina


Abstract

A crude mosquito larvae and dialysed extract alters the mitotic rate of several epithelial cell populations in normal young and adult hepatectomized mice. A crude extract also showed a biphasic effect on the proliferation of human mononuclear cells (MNCs), either stimulating or inhibiting them depending on the dose applied. In the present paper, we assayed the effect of the dialysed mosquito larvae extract and two different protein fractions on human MNCs. Analysis of cell viability after culture indicated that the extract did not have toxic effects. Our results show a dual response of the MNCs to the dialysed, as well as to the protein fraction, with the highest molecular weight inhibiting or stimulating proliferation, depending on the dose applied. The protein fraction with the lowest molecular weight (range between 12–80kDa) showed only an inhibitory effect on cell proliferation.


Key words: mononuclear cells, lymphocytes, cell proliferation, mosquito, growth factors.

f1To whom correspondence should be addressed. E-mail:jronderos@museo.fcnym.unlp.edu.ar


Received 3 June 1999; accepted 20 November 1999

doi:10.1006/cbir.1999.0489


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)