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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2000) 24, 279–283 (Printed in Great Britain)
EFFECT OF SERUM WITHDRAWAL ON THE PROLIFERATION OF B16F10 MELANOMA CELLS
Clara Rodriguez‑Ayerbe and Isabel Smith‑Zubiagaf1
Department of Zoology and Animal Cell Dynamic, Faculty of Science, University of the Basque Country, P.O. Box 644, 48080, Bilbao, Spain


Abstract

B16F10 murine melanoma cell proliferation was inhibited after 48h in medium with serum in the range 0.1 to 0.5% by volume. Cell viability was mostly retained, whereas cells completely deprived of serum died. Growth-arrested cultures showed serum-dependent suppression of DNA synthesis. The response was typically that of a ‘cell cycle freeze’, verified by flow cytometric distribution of cells. Consequently, serum deprivation did not lead to synchony when serum was restored to arrested populations. Furthermore, there was no change in PCNA expression in arrested cells.


Key words: B16F10 melanoma cells, serum deprivation, PCNA, BrdU, flow cytometry, cell cycle.

f1To whom correspondence should be addressed. Tel.: 34-4-6012691; fax: 34-4-4648500; E-mail:zopsmzui@lg.ehu.es


Received 1 February 1999; accepted 21 December 1999

doi:10.1006/cbir.1999.0502


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)