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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2000) 24, 351–358 (Printed in Great Britain)
INTERACTION OF PLANT POLYSOMES WITH THE ACTIN CYTOSKELETON
N. Klyachkof1, L. Aksenova, M. Dunaeva and A. Kulikova
Timiryazev Institute of Plant Physiology, Russian Academy of Science, Botanicheskaya ul. 35, Moscow, 127276, Russia


Abstract

Protein composition and functional activity of various polysome subpopulations isolated from Vicia faba L. leaves and Triticum aestivum L. and Hordeum vulgare L. seedlings were studied. Membrane- and cytoskeleton-bound polysomes were more active in the wheat germ cell-free translational system than free polysomes. Several non-ribosomal proteins were detected in the polysome preparations by gel electrophoresis and Western blot analysis: (1) a canonical actin of mol wt 42kDa; (2) a 40kDa protein, demonstrating affinity for ribosomes, sharing some determinants with actin, and present predominantly in the subpopulations of bound polysomes; and (3) an acidic ribosome-associated p40 evenly distributed between free and bound polysomes. The possibility of involvement of these proteins in interactions between polysomes and the actin cytoskeleton is discussed.


Key words: polysome subpopulations, actin cytoskeleton, non-ribosomal proteins, cell-free translation.

f1To whom correspondence should be addressed. E-mail:ifr@ippras.ru


Received 10 June 1999; accepted 20 August 1999

doi:10.1006/cbir.1999.0517


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)