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Cell Biology International (2000) 24, 863–870 (Printed in Great Britain)
EFFECTS OF THE MYOSIN INHIBITOR 2,3-BUTANEDIONE MONOXIME (BDM) ON CELL SHAPE, LOCOMOTION AND FLUID PINOCYTOSIS IN HUMAN POLYMORPHONUCLEAR LEUCOCYTES
N. Urwylera, P. Egglib and H.U. Kelleraf1
aInstitute of Pathology, University of Bern, Bern, Switzerland
bInstitute of Anatomy, University of Bern, Bern, Switzerland


Abstract

We investigated the role of myosin in polymorphonuclear leucocyte (PMN) shape changes, locomotion, and fluid pinocytosis using the myosin inhibitor 2,3 butanedione monoxime (BDM). Treatment of resting spherical PMNs with BDM produced spheroid cells showing small continuous shape changes (IC50=15.5m m BDM) and occasionally small blebs. Cell polarity, as induced by the chemotactic peptide fNLPNTL or by colchicine, and locomotion were completely suppressed (IC50=8.4 to 10m m). Suppression of fNLPNTL- or colchicine-induced cell polarity produced spheroid cells, suppression of PMA-induced shape changes and fluid pinocytosis produced non-motile spherical cells (IC50=25 to 30m m BDM). BDM suppressed formation of lamellipodia but not formation of blebs. Suppression of microvilli by BDM as observed in resting spherical cells was partially antagonized by PMA. The results suggest that myosin is involved in stabilizing the shape of resting spherical cells, including microvilli, and that myosin is required for cell polarity, locomotion, fluid pinocytosis and for formation of lamellipodia, but not for formation of blebs.


Key words: blebs, colchicine, fNLPNTL, lamellipodia, polarity.

f1To whom correspondence should be addressed: Prof. H.U. Keller, Institute of Pathology, Murtenstrasse 31, CH-3010 Bern, Switzerland. E-mail:kellerhu@patho.unibe.ch


Received 2 March 2000; accepted 26 May 2000

doi:10.1006/cbir.2000.0579


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)