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Cell Biology International (2002) 26, 193201 (Printed in Great Britain)
DEFINITIVE EXPRESSION OF C-MOS IN LATE MEIOTIC PROPHASE LEADS TO PHOSPHORYLATION OF A 34
Division of Clinical Science, Medical Research Institute, and Department of Biochemistry, Kanazawa Medical University, Uchinada, Ishikawa, 920-0293, Japan
To investigate the role of c-mos in rat spermatogenesis, expression of c-mos, MAP kinase kinase (MAPKK), MAP kinase (MAPK), cdc2 and protein kinase A (PKA) by spermatogenic cell culture of 14 day-old rats was examined. MAPKK and PKA expressions were constitutive, whereas the expression of MAPK and cdc2 in spermatogonia initially decreased, but later increased on meiotic maturation of spermatocytes. c-mos expression was definitive of late meiotic prophase. c-mos immunoprecipitates prepared from the c-mos-enriched fraction (pI
Key words: spermatogenesis, c-mos, MAP kinase kinase, MAP kinase, cdc2, protein kinase A.
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Received 3 April 2001; accepted 12 September 2001doi:10.1006/cbir.2001.0834