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Cell Biology International (2003) 27, 23–29 (Printed in Great Britain)

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Changes in lymphokine receptor expression and fatty acid composition of phospholipids and triacylglycerols in rat adipocytes associated with lymph nodes following a transient immune challenge

J.D. Priddle^*, C.A. Mattacks, D.A. Sadler, H.A. MacQueen and C.M. Pond

Department of Biological Sciences, The Open University, Milton Keynes MK7 6AA, UK

Abstract

Single-photon counting fluorimetry was used to record the time course of the expression of interleukin-10 receptors labelled with fluorescent antibodies on the surface of adipocytes over 24h, following an immune challenge to the rat popliteal lymph node. Homologous perinodal and remote-from-node samples from the stimulated and unstimulated popliteal depots were compared in rats fed on plain chow and chow supplemented with 10% w/w suet, fish or vegetable oils. Receptor expression was maximal 6h after stimulation, and returned to baseline after 24h, and was similar in the stimulated and unstimulated depots. Fewer receptors were elicited in tissues from rats fed lipid-supplemented diets compared with the control diet, with fewest of all following the fish oil diet. These data suggest that interleukin-10 is involved in local interactions between perinodal adipocytes and lymph node lymphoid cells. Both triacylglycerols and phospholipids contained more polyunsaturates and fewer saturates in perinodal adipose tissue than in samples from sites not associated with lymphoid tissue. These data are consistent with paracrine interactions between perinodal adipocytes and activated lymphoid cells.

Key words: Interleukin-10 receptors, Phospholipids, Site-specific properties, Perinodal adipose tissue, Lymph nodes.

^*Corresponding author. Fax: +44-1908-654167

Received 4 April 2002; accepted 17 September 2002