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Cell Biology International (2003) 27, 785–789 (Printed in Great Britain)

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Vacuolar accumulation and extracellular extrusion of electrophilic compounds by wild-type and glutathione-deficient mutants of the methylotrophic yeast Hansenula polymorpha

Vira M. Ubiyvovk¹, Janusz Maszewski², Grzegorz Bartosz²³ and Andrei A. Sibirny¹³*

¹Institute of Cell Biology, National Academy of Sciences of Ukraine, Drahomanov Street, 14/16, Lviv 79005, Ukraine
²Department of Molecular Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland
³Rzeszów University, Cegielniana 12, 35-310 Rzeszów, Poland

Abstract

The methylotrophic yeast Hansenula polymorpha CBS4732 leu2 detoxifies electrophilic xenobiotics by glutathione (GSH)-dependent accumulation in vacuoles, as shown by fluorescence microscopy. GSH-dependent and GSH-independent export of xenobiotic derivatives were also demonstrated by high-performance liquid chromatography (HPLC). Conjugates of GSH and N-acetylcysteine with monobromobimane and N-[1-pyrene]maleimide were observed among the HPLC fractions, along with unidentified derivatives.

Key words: Glutathione, Methylotrophic yeast, Hansenula polymorpha, Xenobiotics, Detoxification, Accumulation, Export.

^*Corresponding author. Tel.: +380-322-740-363; fax: +380-322-721-648

Received 11 February 2003/27 March 2003; accepted 13 May 2003