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Cell Biology International (2003) 27, 791794 (Printed in Great Britain)
Apoptotic effect of gossypol on human lymphocytes
Erkan Yurtcu1, Mehmet Ali Ergun2 and Adnan Menevse2*
1Hematology Laboratory, Gazi University, 06510, Besevler, Ankara, Turkey
2Department of Medical Biology and Genetics, Gazi University, 06510, Besevler, Ankara, Turkey
The overall objective of this study was to determine the effect of gossypol on human lymphocytes. Blood samples were taken from healthy donors and lymphocytes were cultured with various concentrations of gossypol (25–150 μM). The percentage of apoptotic cells was determined by assessing DNA fragmentation by agarose gel electrophoresis; morphological features of apoptosis were assessed by light microscopy. The concentrations of gossypol required to induce apoptosis in human lymphocytes without causing necrosis through cytotoxic effects were between 25–50 μM.
Key words: Gossypol, Apoptosis, Human lymphocyte.
*Corresponding author. Tel.: +90-312-214-10-00x6932; fax: +90-312-212-46-47
Received 6 January 2003/8 May 2003; accepted 14 July 2003doi:10.1016/S1065-6995(03)00168-9