|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2003) 27, 913919 (Printed in Great Britain)
Sulfonated human immunoglobulin enhances CD16-linked CD11b expression on human neutrophils
Hirokazu Kimuraa*1, Masahiko Katob1, Miyuki Ikedaa, Akira Nagaia, Yasunori Okadab, Shigeto Naitoc, Shigeru Oshimac, Koichi Taniguchic, Kunihisa Kozawaa and Akihiro Morikawab
aGunma Prefectural Institute of Public Health and Environmental Sciences, 378, Kamioki, Maebashi, Gunma 371-0052, Japan
bDepartment of Pediatrics, Gunma University, School of Medicine, Maebashi, Gunma, Japan
cGunma Prefectural Cardiovascular Center, Maebashi, Gunma, Japan
Intravenous human immunoglobulin therapy infrequently results in excessive inflammatory responses in vivo; these effects are not fully understood. We assessed whether sulfonated human immunoglobulin (SHIG) or polyethylene glycol-treated human immunoglobulin (PHIG) enhanced expression of inflammatory receptors on peripheral blood neutrophils in vitro, such as αMβ2 (CD11b/CD18) and Fc gamma receptor type III (FcγRIII). CD11b and CD16 expression on neutrophils was measured by fluorescence flow cytometry. Various cytokines were assessed using a highly sensitive fluorescence microsphere system. SHIG enhanced/induced CD11b expression and partial aggregations on neutrophils, but PHIG did not. No detection of aggregation IgG was observed in SHIG and PHIG. SHIG-induced CD11b expression was inhibited by treatment of corticosteroid (dexamethasone) and by anti-CD16 monoclonal antibody. Concentrations of various cytokines such as interleukin (IL)-1β, IL-2, IL-4, IL-5, IL-6, IL-8, IL-10, RANTES, tumor necrosis factor (TNF)-α, and interferon (INF)-γ in culture supernatant were not significantly changed by SHIG or PHIG. SHIG and PHIG did not enhance CD16 on neutrophils. SHIG enhanced CD16-linked CD11b expression on neutrophils in vitro. CD11b induction was inhibited by dexamethasone and by anti-CD16 antibody. These in vitro results suggest that aggregations and enhancement of CD11b on neutrophils by SHIG may induce excessive inflammatory responses in vivo.
Key words: Sulfonated human immunoglobulin, Neutrophils, CD11b, CD16, Corticosteroid.
1H. Kimura and M. Kato contributed equally to this work
*Corresponding author. Tel.: +81-27-232-4881; fax: +81-27-234-8438
Received 27 January 2003/12 May 2003; accepted 31 July 2003doi:10.1016/j.cellbi.2003.07.003