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Cell Biology International (2004) 28, 237242 (Printed in Great Britain)
Apoptosis and nitric oxide release induced by thalidomide, gossypol and dexamethasone in cultured human chronic myelogenous leukemic K-562 cells
Mehmet Ali Erguna, Ece Konaca, Deniz Erbasb and Abdullah Ekmekcia*
aDepartment of Medical Biology and Genetics, Faculty of Medicine, Gazi University, Besevler, Ankara 06510, Turkey
bDepartment of Physiology, Faculty of Medicine, Gazi University, Besevler, Ankara, Turkey
The effects of different dosages of thalidomide, gossypol and dexamethasone on the levels of apoptosis and nitric oxide (NO) production were studied in a human chronic myelogenous leukemic cell line, K-562. Increases in the levels of apoptosis were induced by both 15 and 30 μM of thalidomide but only 15 μM significantly increased NO production. All dosages of gossypol used increased the production of NO and all dosages except 200 μM raised the level of apoptosis. After dexamethasone treatment the level of NO either decreased or stayed constant. Thus, some dosages of thalidomide and gossypol concomitantly raise the levels of apoptosis and NO production, but dexamethasone, though it induced apoptosis, had no significant effect on NO production.
Key words: Apoptosis, Nitric oxide, Thalidomide, Gossypol, Dexamethasone, K-562.
*Corresponding author. Tel.: +90-312-214-10-81; fax: +90-312-212-46-47
Received 23 October 2003/20 November 2003; accepted 8 December 2003doi:10.1016/j.cellbi.2003.12.005