|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2004) 28, 361371 (Printed in Great Britain)
Hepatocyte growth factor disrupts tight junctions in human breast cancer cells
Tracey A Martin*, Gareth Watkins, Robert E Mansel and Wen G Jiang
Metastasis Research Group, University Department of Surgery, University of Wales College of Medicine, Heath Park, Cardiff CF14 4XN, UK
Tight junctions in epithelial cells act as cell–cell adhesion structures and govern paracellular permeability. Disruption of these functions can lead to dissociation of cancer cells. This study aimed to determine whether HGF, a cytokine secreted by stromal cells, is capable of modulating expression and function of tight junction molecules in human breast cancer cell lines. HGF decreased trans-epithelial resistance and increased paracellular permeability of two human breast cancer cell lines, MDA MB 231 and MCF-7. Q-PCR showed that HGF modulated the levels of several tight junction molecule (occludin, claudin-1 and -5, JAM-1 and -2) mRNA transcripts in MDA MB 231 and MCF-7 cells. Western blotting and immunohistochemistry also showed modulation of expression of the tight junction molecule, occludin. It is suggested that HGF disrupts tight junction function in human breast cancer cells by effecting changes in the expression of tight junction molecules at both the mRNA and protein levels. We conclude that regulation of tight junctions could be of fundamental importance in the prevention of metastasis of breast cancer cells.
Key words: Tight junctions, HGF, Breast cancer, Metastasis.
*Corresponding author. Tel.: +44-29-20744711; fax: +44-29-20761623.
Received 20 May 2003/10 February 2004; accepted 11 March 2004doi:10.1016/j.cellbi.2004.03.003