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Cell Biology International (2004) 28, 449–455 (Printed in Great Britain)
A small heat-shock protein, p26, from the crustacean Artemia protects mammalian cells (Cos-1) against oxidative damage
Crista H Collinsa and James S Cleggab*
aBodega Marine Laboratory, University of California (Davis), Bodega Bay, CA 94923, USA
bSection of Molecular and Cellular Biology, University of California (Davis), Bodega Bay, CA 94923, USA


Abstract

A small heat-shock protein (p26) purified from stress-resistant embryos of the crustacean, Artemia franciscana, was introduced into cultured cells of green monkey kidney (Cos-1) using the BioPORTER® delivery system. Cells containing p26 exhibited impressive resistance to hydrogen peroxide compared to controls. Introduction of the disaccharide trehalose did not provide protection against oxidative damage, but enhanced substantially the protective performance of p26 when both were present. These studies extend previous research on the protective role played by p26 in cells exposed to various forms of stress, presumably through its ability to function as a molecular chaperone.


Key words: p26, Cos-1 cells, Artemia, H2O2, BioPORTER®.

*Corresponding author. Section of Molecular and Cellular Biology, University of California (Davis), Bodega Bay, CA 94923, USA. Tel.: +1-707-875-2919; fax: +1-707-875-2009.


Received 11 August 2003/27 November 2003; accepted 24 March 2004

doi:10.1016/j.cellbi.2004.03.014


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)