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Cell Biology International (2004) 28, 463–469 (Printed in Great Britain)

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Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells

K Katoh^ab*, A Matsuda^ab, A Ishigami^ab, S Yonekura^ab, H Ishiwata^ab, C Chen^ab and Y Obara^ab

^aDepartment of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
^bPrince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, Victoria 3168, Australia

Abstract

We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca²⁺]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10⁻⁶ to 10⁻³M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10⁻⁴M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10⁻⁸M)-induced increases in [cAMP]c and [Ca²⁺]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.

Key words: Bisphenol A, GH release, Sheep, Anterior pituitary cells, Cyclic AMP, Cytosolic calcium, GHmRNA.

^*Corresponding author. Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. Tel.: +81-22-717-8700; fax: +81-22-717-8701.

Received 28 August 2003; accepted 25 March 2004