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Cell Biology International (2004) 28, 463–469 (Printed in Great Britain)
Suppressing effects of bisphenol A on the secretory function of ovine anterior pituitary cells
K Katohab*, A Matsudaab, A Ishigamiab, S Yonekuraab, H Ishiwataab, C Chenab and Y Obaraab
aDepartment of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan
bPrince Henry's Institute of Medical Research, P.O. Box 5152, Clayton, Victoria 3168, Australia


Abstract

We investigated the action of bisphenol A (BPA) on cellular GH release and content, cell number, GHmRNA expression, and concentrations of cellular cyclic AMP ([cAMP]c) and calcium ion ([Ca2+]c) in primary cultured ovine anterior pituitary cells. The following results were found: (1) BPA as well as nonylphenol (NP) at 10−6 to 10−3M significantly and concentration-dependently suppressed basal and GHRH-stimulated GH release, and the cellular GH content, (2) BPA suppressed the cell number in a time- and concentration-dependent manner, (3) 10−4M BPA suppressed GHmRNA expression to 68% of control (BPA-free), and abolished GHRH (10−8M)-induced increases in [cAMP]c and [Ca2+]c. From these findings we conclude that BPA possesses a suppressing action on GH synthesis and release, and this suppressing action is probably related to impairment of cellular signal transduction systems in ovine anterior pituitary cells.


Key words: Bisphenol A, GH release, Sheep, Anterior pituitary cells, Cyclic AMP, Cytosolic calcium, GHmRNA.

*Corresponding author. Department of Animal Physiology, Graduate School of Agricultural Science, Tohoku University, Amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. Tel.: +81-22-717-8700; fax: +81-22-717-8701.


Received 28 August 2003; accepted 25 March 2004

doi:10.1016/j.cellbi.2004.03.016


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)