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Cell Biology International (2004) 28, 549555 (Printed in Great Britain)
Altered cyclic expression of epithelial Na+ channel subunits and cystic fibrosis transmembrane conductance regulator in mouse endometrium by a low sodium diet
Lai Ling Tsang, Ling Nga Chan and Hsiao Chang Chan*
Epithelial Cell Biology Research Center, Department of Physiology, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, Hong Kong
Epithelial Na+ channel (ENaC) and cystic fibrosis transmembrane conductance (CFTR) have been shown to exhibit cyclic expression patterns in the uterus and demonstrated to play important roles in regulating uterine fluid absorption and secretion. The present study investigated the effect of a low Na+ diet on the cyclic expression of uterine ENaC subunits and CFTR in mice. Ten to 12 weeks old ICR mice with synchronized estrus cycle were fed with a low sodium diet for at least 2 weeks and the mRNA level of these ion channels was examined by semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR). Functional channel activities in primary cultures of endometrial epithelia were assessed by the short-circuit current (I
Key words: CFTR, ENaC, Endometrium, Low Na+ diet, Aldosterone, RT-PCR.
*Corresponding author. Tel.: +852-2609-6839; fax: +852-2603-5022.
Received 30 December 2003/18 March 2004; accepted 26 April 2004doi:10.1016/j.cellbi.2004.04.011