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Cell Biology International (2005) 29, 559566 (Printed in Great Britain)
Characterization of ATP and ADP hydrolysis activity in rat gastric mucosa
Lucielli Savegnago, Cristina W. Nogueira, Roselei Fachinetto and Joao Batista Teixeira Rocha*
Departamento de Quimica, Centro de Ciencias Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, CEP 97105-900, RS, Brazil
The degradation of nucleotides is catalyzed by the family of enzymes called nucleoside triphosphate diphosphohydrolases (NTPDases). The aim of this work was to demonstrate the presence of NTPDase in the rat gastric mucosa. The enzyme was found to hydrolyze ATP and ADP at an optimum pH of 8.0 in the presence of Mg2+ and Ca2+. The inhibitors ouabain (0.01–1 mM), N-ethylmaleimide (0.01–4 mM), levamisole (0.10–0.2 mM) and Ap
Key words: NTPDase, Stomach, Extracellular ATP, Ectonucleotidases, Apyrase.
*Corresponding author. Tel.: +55 55 220 8140; fax: +55 55 220 8978.
Received 16 September 2004/19 January 2005; accepted 8 March 2005doi:10.1016/j.cellbi.2005.03.010