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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2005) 29, 593–597 (Printed in Great Britain)
Identification and expression of the amphioxus Cks1 gene
Hui Yanga1, Zhong‑Ke Chena1, Xiao‑Hui Zhanga, Xiang‑Wei Huanga, Pei‑Jun Zhangb and Hong‑Wei Zhanga*
aSchool of life science, Shandong University, Jinan 250100, China
bInstitute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China


Abstract

A full-length Cks1 homologue gene, AmphiCks1, was identified in amphioxus, Branchiostoma belcheri tsingtauense. Sequence characteristics, phylogeny and patterns of expression during embryonic and larval development were established. The protein predicted from AmphiCks1 showed high sequence identity with vertebrate and invertebrate homologues. Protein structural studies and phylogenetic analysis suggested that Cks homologues are evolutionarily conserved. The AmphiCks1 transcript was detected in most early developmental stages by northern blotting and whole-mount in situ hybridization, suggesting a role for the gene in cell division.


Key words: AmphiCks1, Amphioxus, Cell division, Evolution, Gene expression.

1These authors contributed equally to the study.

*Corresponding author. Tel.: +86 531 8364935; fax: +86 531 8565610.


Received 15 October 2004/20 February 2005; accepted 15 March 2005

doi:10.1016/j.cellbi.2005.03.012


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)