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Cell Biology International (2006) 30, 248–252 (Printed in Great Britain)
Cytotoxicity, haematotoxicity and genotoxicity of high molecular mass arborescent polyoxyethylene polymers with polyglycidol-block-containing shells
Barbara Klajnerta, Wojciech Walachb, Maria Bryszewskaa*, Andrzej Dworakb and Dzmitry Shcharbinc
aDepartment of General Biophysics, University of Lodz, 12/16 Banacha St., 90-237 Lodz, Poland
bInstitute of Coal Chemistry, Polish Academy of Science, 3 Sowinskiego St., 44-121 Gliwice, Poland
cInstitute of Biophysics and Cell Engineering, National Academy of Sciences, 220072 Minsk, Belarus


Abstract

We have examined the impact on biological systems of some newly synthesised polyoxyethylene polymers using in vitro assays. Toxicity was tested by the 3-[4,5-dimethylthiazole-2-yl]-2,5 diphenyltetrazolium bromide (MTT) assay, haemolysis was assessed, and an ethidium bromide (EB) assay was used to study interactions between the polymers and DNA. All the assay data showed that the polymers are biocompatible. No differences were found between generations (i.e. macromolecule sizes). The results encourage continuing studies on the clinical use of these molecules as drug carriers.


Key words: Hyperbranched polymers, Polyoxyethylene, Polyglycidol, MTT assay, Haemolysis, DNA intercalation, Ethidium bromide.

*Corresponding author. Tel./fax: +48 42 635 44 74.


Received 25 November 2004/8 October 2005; accepted 31 October 2005

doi:10.1016/j.cellbi.2005.10.026


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)