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Cell Biology International (2006) 30, 645–652 (Printed in Great Britain)

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Combined effect of low-power laser irradiation and anthraquinone anticancer drug aclarubicin on survival of immortalized cells: Comparison with mitoxantrone

Aneta Koceva‑Chyła^a*, Bogusława Wiēcławska^a, Zofia Jóźwiak^a and Maria Bryszewska^b

^aDepartment of Thermobiology, Institute of Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland
^bDepartment of General Biophysics, Institute of Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland

Abstract

The photodynamic response of the anthraquinone anticancer drug aclarubicin (ACL) was investigated in vitro and compared with that of mitoxantrone (MTX). Cultured immortalized rodent B14 and NIH 3T3 cells were used in the experiments as a model for cells with neoplastic phenotype. Long-term cytotoxicity and inhibition of cell proliferation assayed by the clonal growth and MTT-tetrazolium methods were estimated to compare the efficacy of aclarubicin and mitoxantrone in photosensitizing cells and their death after non-thermal exposure to monochromatic laser light. Green He–Ne (543.5nm) or red semiconductor (670nm) low-power laser (LPL) irradiations were applied. Different dose-responses of both cell lines to aclarubicin and mitoxantrone were found so that the cytotoxicity of MTX was considerably greater than the cytotoxicity of ACL. Phototherapy response (P<0.0001) was observed only for B14 cells after sensitisation with aclarubicin. Under the same conditions no significant effect of red light irradiation (semiconductor 670nm laser) on survival of both cell lines treated with mitoxantrone was found.

Key words: Photodynamic therapy (PDT), Laser, Anthracyclines, Aclarubicin, Mitoxantrone, Cell survival, MTT assay.

^*Corresponding author. Tel./fax: +48 42 635-4473.

Received 26 January 2006/8 March 2006; accepted 3 April 2006