|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2006) 30, 645652 (Printed in Great Britain)
Combined effect of low-power laser irradiation and anthraquinone anticancer drug aclarubicin on survival of immortalized cells: Comparison with mitoxantrone
Aneta Koceva‑Chyłaa*, Bogusława Wiēcławskaa, Zofia Jóźwiaka and Maria Bryszewskab
aDepartment of Thermobiology, Institute of Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland
bDepartment of General Biophysics, Institute of Biophysics, University of Łódź, Banacha 12/16, 90-237 Łódź, Poland
The photodynamic response of the anthraquinone anticancer drug aclarubicin (ACL) was investigated in vitro and compared with that of mitoxantrone (MTX). Cultured immortalized rodent B14 and NIH 3T3 cells were used in the experiments as a model for cells with neoplastic phenotype. Long-term cytotoxicity and inhibition of cell proliferation assayed by the clonal growth and MTT-tetrazolium methods were estimated to compare the efficacy of aclarubicin and mitoxantrone in photosensitizing cells and their death after non-thermal exposure to monochromatic laser light. Green He–Ne (543.5
Key words: Photodynamic therapy (PDT), Laser, Anthracyclines, Aclarubicin, Mitoxantrone, Cell survival, MTT assay.
*Corresponding author. Tel./fax: +48 42 635-4473.
Received 26 January 2006/8 March 2006; accepted 3 April 2006doi:10.1016/j.cellbi.2006.04.003