|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2006) 30, 714720 (Printed in Great Britain)
Effects of fluid shear stress on mRNA expression of carbonic anhydrase II in polarized rat osteoclasts
Qinghong Zhanga, Xing Lianga*, Baoming Zhua, Qiang Donga, Ling Xua, Lu Xiaa, Jian Hua, Jun Fua and Mengtao Liub
aKey Laboratory of Oral Biomedical Engineering of Ministry of Education, West China College of Stomatology, Sichuan University, Chengdu 610041, China
bDepartment of Prosthetics, The Red Cross Hospital of Kunming, Yunnan 650021, China
The present study was designed to determine the effects of fluid shear stress on the mRNA expression of carbonic anhydrase II (CAII) in polarized rat osteoclasts. Cellular morphology of the polarized osteoclasts generated by a mechanical anatomical technique was examined by tartrate-resistant acid phosphatase (TRAP) staining and the osteoclastic resorption of dentine slices. The polarized osteoclasts were then stress-loaded by using a flow shear stress device newly developed by the osteoclast research group (patent number 200420034438; China), at 9
Key words: Osteoclast, Fluid shear stress, Carbonic anhydrase II, Morphology.
*Corresponding author. Tel./fax: +86 28 85503570.
Received 28 October 2005/18 March 2006; accepted 3 May 2006doi:10.1016/j.cellbi.2006.05.002