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Cell Biology International (2006) 30, 784–792 (Printed in Great Britain)

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Contribution of α2β1, α3β1, α6β4 integrins and 67kDa laminin receptor to the interaction of epidermoid carcinoma A-431 cells with laminin-2/4

Olga A. Cherepanova^*1, Natalia Kalmykova, Yury P. Petrov, Miralda Blinova and George Pinaev

Department of Cell Culture, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia

Abstract

Laminin-2/4 is the major laminin isoform of normal muscle and nerve tissues and plays an important role in tumor invasion and metastasis. Despite the fact that laminin-2/4 has been found in the skin basement membrane, insufficient evidence is available on the effect of laminin-2/4 on the behavior of both normal and transformed skin cells. A comparison of the contribution of α2β1, α3β1, α6β4 integrins and 67kDa laminin receptor on the surface of the human epidermoid carcinoma cell, A-431, to interaction with laminin-2/4 was carried out. The cell interaction with extracellular matrix component is a multistage process. We employed new methods for studying different stages of the interaction of A-431 cells with laminin-2/4. We demonstrated that integrins α2β1, α3β1, α6β4 and 67kDa laminin receptor are involved in the interaction of A-431 cells with laminin-2/4. We found that contribution of the same receptors to different stages of the interaction with laminin can be different. α2β1 integrins are involved in EGF-induced A-431 cells' migration on laminin-2/4. We demonstrated the cooperation between α2β1 and α3β1 integrins during adhesion and spreading of A-431 cells on laminin-2/4-coated substrate. These results provide information about laminin-2/4 receptors and their contribution to different stages of the interaction with cells.

Key words: Laminin-2/4, Integrins, 67kDa Laminin receptor, A-431 cells.

¹Contract grant sponsor: Russian Foundation for Basic Research; Contract grant number: 03-04-48251.

^*Corresponding author. Cardiovascular Research Center, 415 Lane Road, MR5 RM 1328, Charlottesville, VA 22908, USA. Tel.: +1 434 924 5993; fax: +1 434 982 0055.

Received 4 January 2006/4 May 2006; accepted 23 June 2006