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Cell Biology International (2006) 30, 861865 (Printed in Great Britain)
Perinuclear localization of biogenic amines (serotonin and histamine) in rat immune cells
G. Csaba* and P. Kovács
Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvárad tér 4, 1445 Budapest, Hungary
EDAC fixation, which polymerizes biogenic amines, and clorgyline, which blocks monoamine oxydase, were used to demonstrate the perinuclear and nuclear localization of histamine and serotonin (5-HT) of immune cells in rat peritoneal fluid and thymus. For detection, an immunocytochemical method combined with confocal microscopy was used. In peritoneal cells (lymphocytes) 5-HT formed spots around the nucleus, while histamine was localized diffusely. In thymocytes, 5-HT was distributed in patches, while histamine formed a garland around the nuclear envelope. Clorgyline enhanced the fluorescence and increased the number of fluorescent positive cells only for histamine, and then histamine-positive nuclei were also present. The results show that: (1) EDAC fixation demonstrates the presence of biogenic amines in immune cells, (2) using this fixation, the perinuclear localization of biogenic amines can be demonstrated, (3) the localization of the two amines is different and (4) the nuclear localization of histamine in thymocytes can be surmised and in mast cells (described previously) is proven again. The potential importance of the nuclear and near-nuclear localization of biogenic amines is discussed.
Key words: Serotonin, Histamine, Lymphocyte, Thymus, EDAC fixation.
*Corresponding author. Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvárad tér 4, P.O. Box 370, 1445 Budapest, Hungary. Tel./fax: +36 1 210 2950.
Received 6 January 2006/27 April 2006; accepted 9 June 2006doi:10.1016/j.cellbi.2006.06.021