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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2006) 30, 866–878 (Printed in Great Britain)
αII-Spectrin interacts with five groups of functionally important proteins in the nucleus
Deepa M. Sridharan, Laura W. McMahon and Muriel W. Lambert*
Department of Pathology and Laboratory Medicine, UMDNJ – New Jersey Medical School and the Graduate School of Biomedical Sciences, Newark, NJ 07103, USA


Abstract

Nonerythroid α-spectrin (αSpIIΣ*) is a structural protein that has been identified in the nucleus of mammalian cells and shown to be involved in DNA repair. It is also deficient in cells from the clinically diverse genetic disorder Fanconi anemia (FA). In order to get a clearer understanding of the role of αSpIIΣ* in DNA repair, and whether it may have other important functions in the nucleus, studies were undertaken to identify specific αSpIIΣ* protein binding partners in the nucleus. The results demonstrate that multiple proteins co-immunoprecipitate with αSpIIΣ* from nuclear extracts from normal human lymphoblastoid and HeLa cells. These can be grouped into five categories: structural proteins, proteins involved in DNA repair, chromatin remodeling proteins, FA proteins, and transcription and RNA processing factors. These studies indicate that αSpIIΣ* may play a role in a number of diverse and important processes in the nucleus and that a deficiency in this protein, as occurs in FA, could affect a number of critical cellular pathways.


Key words: Nonerythroid α-spectrin, Structural proteins, Fanconi anemia proteins, DNA repair proteins, Chromatin remodeling proteins, Transcription and RNA processing proteins.

*Corresponding author. Tel.: +1 973 972 4405; fax: +1 973 972 7293.


Received 19 May 2006; accepted 2 June 2006

doi:10.1016/j.cellbi.2006.06.005


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)