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Cell Biology International (2006) 30, 947951 (Printed in Great Britain)
Nitric oxide-independent lipid metabolism in RAW 264.7 macrophages loaded with oleic acid
Wen‑Chen Huanga and Jin‑Shan Chenb*
aDivision of Plastic Surgery, Department of Surgery, Mackay Memorial Hospital, Taipei 110, Taiwan, ROC
bDepartment of Anatomy, Taipei Medical University, 250 Wu-Hsing Street, Taipei 110, Taiwan, ROC
The role of nitric oxide (NO) in the regulation of lipogenesis and lipolysis in RAW 264.7 macrophages loaded with oleic acid (OA) was investigated in this paper. Magnolol stimulated full lipolysis without affecting NO levels. Both inhibition and elevation of NO production in OA-loaded macrophages did not induce lipolysis. Besides, lipopolysaccharide (LPS)-induced increased accumulation of lipid droplets was not reduced by down-regulation of NO levels. Moreover, incubation of macrophages with sodium nitroprusside (SNP), an NO donor, stimulated significant NO production without altering the lipid droplet accumulation. All these results clearly demonstrate that NO is not involved in the modulation of lipid metabolism in macrophages loaded with OA.
Key words: Macrophage, Oleic acid, Nitric oxide, Lipid droplet, Lipogenesis, Lipolysis, Lipid metabolism.
*Corresponding author. Tel.: +886 2 27361661; fax: +886 2 27388852.
Received 25 April 2006/2 June 2006; accepted 26 June 2006doi:10.1016/j.cellbi.2006.06.014