|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2007) 31, 2429 (Printed in Great Britain)
Nitric oxide affects preimplantation embryonic development in a rotating wall vessel bioreactor simulating microgravity
Yu‑jing Caoa1, Xun‑jun Fanab1, Zheng Shena, Bao‑hua Maac and En‑kui Duana*
aState Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 25 Beisihuan Xi Lu, Haidian, Beijing 100080, People's Republic of China
bGraduate School of the Chinese Academy of Sciences, 19 Yu-quan Road, Beijing 100039, People's Republic of China
cCollege of Animal Science and Technology, Northwest A&F University, 3 Taicheng Road, Yangling, Shaanxi 712100, People's Republic of China
Microgravity was simulated with a rotating wall vessel bioreactor (RWVB) in order to study its effect on pre-implantation embryonic development in mice. Three experimental groups were used: stationary control, rotational control and clinostat rotation. Three experiments were performed as follows. The first experiment showed that compared with the other two (control) groups, embryonic development was significantly retarded after 72
Key words: Simulated microgravity, Embryonic development, Nitric oxide, Nitric oxide synthase, Apoptosis.
1These authors contributed equally to this work.
*Corresponding author. Tel.: +86 10 6255 8112 (Off), +86 10 6263 1831 (Lab); fax: +86 10 6263 1831.
Received 9 February 2006/21 July 2006; accepted 4 September 2006doi:10.1016/j.cellbi.2006.09.003