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Cell Biology International (2007) 31, 119125 (Printed in Great Britain)
Expression of PTEN and Akt phosphorylation in lipopolysaccharide-treated NIH3T3 cells
Hirohiko Okamuraa*, Kaya Yoshidaa, Eiko Sasakia, Lihong Qiua, Bruna Rabelo Amorima, Hiroyuki Morimotob and Tatsuji Hanejia
aDepartment of Histology and Oral Histology, Institute of Health Biosciences, The University of Tokushima Graduate School, 3-18-15 Kuramoto-cho, Tokushima 770-8504, Japan
bDepartment of Oral and Maxillofacial Anatomy, Institute of Health Biosciences, The University of Tokushima Graduate School, Kuramoto, Tokushima 770-8504, Japan
PTEN is a tumor suppressor gene encoding a phosphatase, and it negatively regulates cell survival mediated by the phosphoinositol 3-kinase (PI3-Kinase)-Akt pathway. To elucidate PTEN expression and its effect on the PI3-kinase-Akt pathway in fibroblasts and macrophages, we investigated the expression of PTEN and the phosphorylation status of Akt in NIH3T3 and RAW264.7 cells treated with LPS. Phosphorylation of Akt was induced by LPS treatment in a dose-dependent manner in RAW264.7 cells, but not in NIH3T3 cells. LPS induced the expression of PTEN in a dose and time-dependent manner in NIH3T3 cells (0–1
Key words: Akt, Fibroblast, Lipopolysaccharide, Phosphorylation, PTEN.
*Corresponding author. Tel.: +81 88 633 7322; fax: +81 88 633 7342.
Received 15 February 2006/31 July 2006; accepted 14 September 2006doi:10.1016/j.cellbi.2006.09.014