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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2007) 31, 444–450 (Printed in Great Britain)
Ex vivo expansion, adipogenesis and neurogenesis of cryopreserved human bone marrow mesenchymal stem cells
Ying Xianga, Qiang Zhengb, Bingbing Jiaa, Guoping Huanga, Chungang Xiea, Jianjun Panb** and Jinfu Wanga*
aInstitute of Cell Biology, College of Life Sciences, Zhejiang University, 232 Wen San Road, Hangzhou, Zhejiang 310012, P.R. China
bThe Second Affiliated Hospital, Zhejiang University, Hangzhou 310006, P.R. China


This study aimed to investigate the potentials of ex vivo expansion and pluridifferentiation of cryopreserved adult human bone marrow mesenchymal stem cells (hMSCs) into adipocytes and neurocytes. Cryopreserved hMSCs were resuscitated and cultured for 15 passages, and then induced to adipocytes and neurocytes with corresponding induction medium. The induced cells were observed for morphological properties and expression of triglyceride or neuron-specific enolase and nestin was detected. The result showed that the resuscitated cells cultured in induction medium consisting of dexamethasone, 3-isobutyl-1-methylxanthine, indomethacin and insulin-like growth factor I (IGF-I) showed adipogenesis, and lipid vacuole accumulation was detectable after 21days. The resuscitated hMSCs were also induced into neurocytes and expressed nestin and neuron-specific enolase (NSE), which are special surface markers associated with neural cells at different stages. This study suggested that resuscitated hMSCs should still be a population of pluripotential cells and should be accessible for establishing an abundant hMSC reservoir for further experiment and treatment of various clinical diseases.

Key words: Bone marrow mesenchymal stem cells, Cryopreservation, Expansion, Differentiation.

*Corresponding author. Tel.: +86 571 8820 6592; fax: +86 571 85128776.

**Corresponding author. Tel.: +86 571 87783542; fax: +86 571 87074866.

Received 10 June 2006/8 October 2006; accepted 5 November 2006


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)