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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2007) 31, 1462–1469 (Printed in Great Britain)
Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells
Noriyoshi Mizuno*, Hideki Shiba, Wan‑peng Xu, Takafumi Inui, Tsuyoshi Fujita, Mikihito Kajiya, Katsuhiro Takeda, Naohiko Hasegawa, Hiroyuki Kawaguchi and Hidemi Kurihara
Department of Periodontal Medicine, Division of Frontier Medical Science, Programs for Biomedical Research, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3, Kasumi, Minami-ku, Hiroshima 734-8553, Japan


Neurotrophins (NTs) are expressed during tooth development. However, little is known about a role of NTs in differentiation of pulp cells into mineralizing cells. In this study, mRNA expressions of hard tissue-related proteins, calcification and proliferation are examined in cultures of human pulp (HP) cells. Nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), neurotrophin (NT)-3 and NT-4/5 increased the mRNA levels of dentin sialophsphoprotein, alkaline phosphatase, osteopontin, type I collagen and bone morphogenetic protein-2 and mineral deposition in cultures of HP cells. The increased levels and manners varied, depending on the concentrations of NTs and hard-tissue related protein tested. On the other hand, only NGF significantly stimulated DNA synthesis in cultures of HP cells. These findings suggest that NTs characteristically regulate hard-tissue related protein expression, calcification and proliferation in pulp cells. NTs may accelerate pulp cell differentiation.

Key words: Neurotrophins, Human pulp cells, Hard tissue-related proteins.

*Corresponding author. Tel.: +81 82 257 5663; fax: +81 82 257 5664.

Received 28 June 2006/30 March 2007; accepted 26 June 2007


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)