|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2008) 32, 210216 (Printed in Great Britain)
Antisense oligonucleotide Elk-1 suppresses the tumorigenicity of human hepatocellular carcinoma cells
Tsung‑Ho Yingab, Yi‑Hsien Hsiehb, Yih‑Shou Hsiehb and Jer‑Yuh Liub*
aDepartment of Obstetrics and Gynecology, Chung Shan Medical University Hospital, No. 110, Sec. 1, Chien-Kuo N. Road, Taichung 40203, Taiwan
bInstitute of Biochemistry and Biotechnology, School of Medicine, College of Medicine, Chung Shan Medical University, No. 110, Sec. 1, Chien-Kuo N. Road, Taichung 40203, Taichung, Taiwan
In previous studies, we showed that reducing Ets-like protein-1 (Elk-1) expression inhibited protein kinase C alpha (PKCα) expression and decreased cell migration and invasion in human hepatocellular carcinoma (HCC). In this study, we have investigated the role of Elk-1 in tumorigenesis. SK-Hep-1 HCC cells were transfected with the ElK-1 antisense oligonucleotide (ODN). In the pretreated cells we detected a reduction of mRNA level using RT–PCR. The inhibitory rate of cell growth was measured by MTT assay. Pretreated-SK-Hep-1 HCC cells were implanted subcutaneously into nude mice to observe the tumor growth and calculate tumor inhibitory rate. The results showed that 5
Key words: Elk-1, Hepatocellular carcinoma, Protein kinase C alpha, Tumorigenesis, Antisense oligonucleotide.
*Corresponding author. Tel.: +886 4 2473 0022x11673; fax: +886 4 2324 8195.
Received 23 April 2007/3 August 2007; accepted 29 August 2007doi:10.1016/j.cellbi.2007.08.027