|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2008) 32, 344352 (Printed in Great Britain)
Effect of uniaxial stretching on rat bone mesenchymal stem cell: Orientation and expressions of collagen types I and III and tenascin-C
Lei Zhangab, Cyril J.F. Kahna, Huai‑Qing Chenb, Nguyen Tranc and Xiong Wanga*
aLEMTA UMR 7563 CNRS-INPL-UHP, Nancy-University, 54500 Vandoeuvre-les-Nancy, France
bInstitute of Biomedical Engineering, West China Medical Center, Sichuan University, 610041 Chengdu, PR China
cLaboratory of Surgery, Faculty of Medicine, Nancy-University, 54500 Vandoeuvre-les-Nancy, France
Bone marrow mesenchymal stem cells (BMSC) have the potential to differentiate into a variety of cell types like osteoblasts, chondroblasts, adipocytes, etc. It is well known that mechanical forces regulate the biological function of cells. The aim of this study was to investigate the effect of uniaxial stretching on the orientation and biological functions of BMSC. Rat BMSCs were harvested from femoral and tibial bone marrow by density gradient centrifugation. Cells from passages 1–6 were characterized by flow cytometry using monoclonal antibodies. The recovered cells were stably positive for the markers CD90 and CD44 and negative for CD34 and CD45. A cyclic 10% uniaxial stretching at 1
Key words: Bone mesenchymal stem cells (BMSC), Uniaxial stretching, Tissue engineering of ligaments, Collagen, Tenascin-C.
*Corresponding author. Tel.: +33 383 595736; fax: +33 383 595544.
Received 2 August 2007/18 September 2007; accepted 24 December 2007doi:10.1016/j.cellbi.2007.12.018