|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2008) 32, 777783 (Printed in Great Britain)
Redistribution of cytosolic FGFR1 after induced migration of urothelial cells in culture
Peter Veraniča*, Barbara Dariša, Daša Zupančiča, Urška Batistab and Kristijan Jezernika
aInstitute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
bInstitute of Biophysics, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
The distribution of cytosolic fibroblast growth factor receptor 1 (FGFR1) was studied in correlation to cell migration in urothelial cell line g/G. Cell motility was analysed with a new method using consecutive series of photographs of cells relocated on CELLocate coverslips and with image analysis software. The results confirmed that FGF1 stimulated cell motility only when cells were grown on collagen I coating. During the transition from sessile to motile cell phenotype a complete redistribution of cytosolic FGFR1 was revealed. In sessile cells, FGFR1 had a filamentous distribution and its location matched cytokeratin 7. In cells of the migrating phenotype, the distribution of FGFR1 was diffuse, mainly located in cytosol. Our data reveal that the location of cytosolic FGFR1 depends on the motile characteristics of the cell. The results also indicate that attachment of cells to collagen I is crucial for the induction of urothelial cell motility with FGF1.
Key words: FGF1, FGF receptor 1, Cytokeratins, Collagen, Cell motility.
*Corresponding author. Tel.: +286 1 5437684; fax: +286 1 5437681.
Received 5 September 2007/16 January 2008; accepted 21 March 2008doi:10.1016/j.cellbi.2008.03.006