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Cell Biology International (2008) 32, 1024–1028 (Printed in Great Britain)

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Mitochondria, endoplasmic reticulum and actin filament behavior after PDT with chloroaluminum phthalocyanine liposomal in HeLa cells

Maíra Maftoum‑Costa^a, Karina Teixeira Naves^a, Alexandre Lima Oliveira^a, Antonio Cláudio Tedesco^b, Newton Soares da Silva^a and Cristina Pacheco‑Soares^a*

^aLaboratory of Cellular Culture and Tecidual Biology – Dynamics of Cellular Compartments, Universidade do Vale do Paraíba – UNIVAP, Av. Shishima Hifumi 2911, 12211-300 São José dos Campos, SP, Brazil
^bDepartment of Chemistry, Faculty of Philosophy Science and Letters, Universidade de São Paulo – USP, Ribeirão Preto, SP, Brazil

Abstract

Photodynamic therapy (PDT) for cancer is a therapeutic modality in the treatment of tumors in which visible light is used to activate a photosensitizer. Cell membranes have been identified as an important intracellular target for singlet oxygen produced during the photochemical pathway. This study analyzed the cytotoxicity in specific cellular targets of a photosensitizer used in PDT in vitro. The photosensitizing effects of chloroaluminum phthalocyanine liposomal were studied on the mitochondria, cytoskeleton and endoplasmic reticulum of HeLa cells. Cells were irradiated with a diode laser working at 670nm, energy density of 4.5J/cm² and power density of 45mW/cm². Fluorescence microscopic analysis of the mitochondria showed changes in membrane potential. After PDT treatment, the cytoskeleton and endoplasmic reticulum presented basic alterations in distribution. The combined effect of AlPHCl liposomal and red light in the HeLa cell line induced photodamage to the mitochondria, endoplasmic reticulum and actin filaments in the cytoskeleton.

Key words: PDT, Fluorescence microscopy, AlPHCl liposomal.

^*Corresponding author. Instituto de Pesquisa & Desenvolvimento – UNIVAP, Av. Shishima Hifumi, 291 Urbanova, 12244-000 São José dos Campos, SP, Brazil. Tel.: +55 12 39471143; fax: +55 12 39471149.

Received 10 December 2007/21 January 2008; accepted 2 April 2008