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Cell Biology International (2008) 32, 14861496 (Printed in Great Britain)
Simultaneous use of electrochemistry and chemiluminescence to detect reactive oxygen species produced by human neutrophils
Sergey Shleevab*, Jonas Wetteröc, Karl‑Eric Magnussond and Tautgirdas Ruzgasa
aBiomedical Laboratory Science, Health and Society, Malmö University, 20506 Malmö, Sweden
bLaboratory of Chemical Enzymology, Institute of Biochemistry, 119071 Moscow, Russia
cRheumatology/AIR, Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden
dMedical Microbiology, Department of Clinical and Experimental Medicine, Linköping University, 58185 Linköping, Sweden
A novel approach for the simultaneous optical and electrochemical detection of biologically produced reactive oxygen species has been developed and applied. The set-up consists of a luminol-dependent chemiluminescence assay combined with two amperometric biosensors sensitive to superoxide anion radicals (O
Key words: Superoxide anion radical, Hydrogen peroxide, Neutrophil, Biosensor, Luminol-dependent chemiluminescence, NADPH oxidase.
*Corresponding author. Biomedical Laboratory Science, Health and Society, Malmö University, 20506 Malmö, Sweden. Tel.: +46 40 665 7414; fax: +46 40 665 8100.
Received 10 January 2008/23 July 2008; accepted 12 August 2008doi:10.1016/j.cellbi.2008.08.016