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Cell Biology International (2009) 33, 65–70 (Printed in Great Britain)

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Different culture conditions used for arresting the G0/G1 phase of the cell cycle in goldfish (Carassius auratus) caudal fin-derived fibroblasts

Casiano H. Choresca^a, Ok Jae Koo^b, Hyun Ju Oh^b, So Gun Hong^b, Dennis K. Gomez^c, Ji Hyung Kim^a, Byeong Chun Lee^b* and Se Chang Park^a*

^aLaboratory of Aquatic Animal Medicine, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea
^bDepartment of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea
^cBrain Korea 21 Program for Veterinary Science, College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea

Abstract

One of the most important factors determining the success of the development of cloned embryos is the cell cycle stage of the donor cells. We investigated the effects of serum starvation, culturing to confluence and roscovitine treatment on the cell cycle synchronization of goldfish caudal fin-derived fibroblasts by flow cytometric analysis. The results show that culturing the cells to confluence (85.5%) and roscovitine treatment (82.71%) yield a significantly higher percentage of cells arrested in the G0/G1 (P<0.05) phase than serum starvation (62.85%). Different concentrations of roscovitine (5, 10, or 15μM) induce cell cycle arrest at the G0/G1 phase.

Key words: Cell cycle synchronization, G0/G1 phase, Goldfish, Serum starvation, Culture to confluence, Roscovitine.

^*Corresponding authors. College of Veterinary Medicine, Seoul National University, Seoul 151-742, South Korea. Tel.: +82 2 880 1282; fax: +82 2 880 1213.

Received 25 March 2008/8 September 2008; accepted 29 September 2008