|Cancer||Cell death||Cell cycle||Cytoskeleton||Exo/endocytosis||Differentiation||Division||Organelles||Signalling||Stem cells||Trafficking|
Cell Biology International (2009) 33, 119124 (Printed in Great Britain)
Effects of Survivin on cell proliferation and apoptosis in MG-63 cells in vitro
Xinjun Lianga1, Mingxu Dab1, Zhiqiang Zhuanga, Weiguang Wua, Zhuang Wua, Yanfeng Wua and Huiyong Shena*
aDepartment of Orthopaedics, Sun-Yat-Sen Memorial Hospital, Sun-Yat-Sen University, 107 West Yanjiang Road, Guangzhou 510120, PR China
bDepartment of General Surgery, Gansu Provincial Hospital, 160 Donggang West Road, Lanzhou 730000, PR China
Osteosarcoma is the most frequent primary malignant tumor of the skeleton and occurs mainly in children and adolescent. The prognosis of osteosarcoma is very poor due to its aggressive and no effective treatment. This study is the first to investigate the anti-cancer effects of antisense pEGFP-C1-Survivin on human osteosarcoma cells. It was shown in our results that Survivin blockaded could significantly induce apoptosis and inhibit the invasive of osteosarcoma cells line MG-63. The effects were probably produced by the decreased expression of Survivin induced by antisense pEGFP-C1-Survivin which was examined by RT-PCR and western blotting. All these suggested that Survivin should be very important in the development of osteosarcoma and Survivin blockaded by using antisense pEGFP-C1-Survivin could markedly inhibit the proliferation and invasion of osteosarcoma cells line MG-63, partially reversed their malignant phenotype. Targeting Survivin might be a promising option in the treatment of osteosarcoma.
Key words: Osteosarcoma, Survivin, Apoptosis.
1These authors contributed equally to this manuscript.
Received 28 June 2008/22 August 2008; accepted 13 October 2008doi:10.1016/j.cellbi.2008.10.013