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Cell Biology International (2009) 33, 578585 (Printed in Great Britain)
Involvement of headless myosin X in the motility of immortalized gonadotropin-releasing hormone neuronal cells
Jun‑Jie Wanga, Xiu‑Qing Fua, Yu‑Guang Guoa, Lin Yuana, Qian‑Qian Gaoa, Hua‑Li Yua, Heng‑Liang Shia, Xing‑Zhi Wanga, Wen‑Cheng Xiongb and Xiao‑Juan Zhua*
aInstitute of Cytology and Genetics, Key Laboratory of Molecular Epigenetics of Ministry of Education, Northeast Normal University, 5268 Renmin Street, Changchun 130024, Jilin, PR China
bProgram of Developmental Neurobiology, IMMAG and Department of Neurology, Medical College of Georgia, Augusta, GA 30912, USA
Myosin X (Myo X), an unconventional myosin with a tail homology 4-band 4.1/ezrin/radixin/moesin (MyTH4-FERM) tail, is expressed ubiquitously in various mammalian tissues. In addition to the full-length Myo X (Myo X FL), a headless form is synthesized in the brain. So far, little is known about the function of this motor-less Myo X. In this study, the role of the headless Myo X was investigated in immortalized gonadotropin-releasing hormone (GnRH) neuronal cells, NLT. NLT cells overexpressing the headless Myo X formed fewer focal adhesions and spread more slowly than the wild-type NLT cells and GFP-expressing NLT cells. In chemomigration assays, the NLT cells overexpressing the headless Myo X migrated shorter distances and had fewer migratory cells compared with the control NLT cells.
Key words: Filopodia, Overexpression, Adhesion, Migration.
*Corresponding author. Tel.: +86 431 85099769; fax: +86 431 85099822.
Received 18 November 2008/16 January 2009; accepted 20 February 2009doi:10.1016/j.cellbi.2009.02.006