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Cancer Cell death Cell cycle Cytoskeleton Exo/endocytosis Differentiation Division Organelles Signalling Stem cells Trafficking
Cell Biology International (2009) 33, 1050–1056 (Printed in Great Britain)
Prognostic implication of CDC25A and cyclin E expression on primary breast cancer patients
Parvin Mehdipoura*, Saeed Pirouzpanaha, Abdolfattah Sarafnejadb, Morteza Atric, S. Tahereh Shahrestanib and Mojgan Haidarib
aDepartment of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Pour Sina Ave., Tehran 1417613151, IR Iran
bDepartment of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran 14155-6446, IR Iran
cDepartment of Surgery, Cancer Institute, Tehran University of Medical Sciences, Tehran 1417613151, IR Iran


Abstract

Defect in cell cycle control is a hallmark character of cancer. We have investigated the association of Ki67 labeling index, cyclin E and CDC25A expressions with clinical follow-up data in breast carcinomas. Flow cytometry was used to detect gene amplification of cyclins in 44 tumor tissue with invasive breast carcinomas. Multivariate Cox proportional hazard ratio test was used to show the correlations. Cyclin E or CDC25A were upregulated in 34% of the tumors. Among the whole total material, expression of cyclin E and of CDC25A were found upregulated in 31.9% and 39.4% of cells, respectively. Both CDC25A and cyclin E protein expression levels were correlated with Ki67 expression level (p<0.001). In addition, the expression of CDC25A was associated significantly with poor survival (P=0.028), whereas no correlation was found with cyclin E. These findings suggest a possible prognostic value for CDC25A as a cell cycle marker and may imply in characteristic of high risk breast cancer patients.


Key words: Breast cancer, Ki 67 labeling index, Cyclin E, CDC25A, Survival.

*Corresponding author. Tel.: +98 9121276727; fax: +98 21 88953005.


Received 18 January 2009/10 May 2009; accepted 15 June 2009

doi:10.1016/j.cellbi.2009.06.016


ISSN Print: 1065-6995
ISSN Electronic: 1095-8355
Published by Portland Press Limited on behalf of the International Federation for Cell Biology (IFCB)