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Figure 2 KLK induces depletion of intracellular Ca2+ stores
(A) Ca2+ influx measurements of T24 cells labelled with Indo1-AM in PBS buffer containing Ca2+ (average of 42 analysed cells). Addition of 100 nmol KLK after 20 s. (B) Same measurements in HBSS buffer without Ca2+ (average of 67 analysed cells). (C) Ca2+ influx measurements of Indo1-AM-loaded T24 cells incubated with the membrane-permeable Ca2+ chelator BAPTA-AM (10 μM). Addition of 100 nmol/ml KLK after 20 s; average of 48 analysed cells.